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Representative microscopic images of <t>Cytokeratin</t> (CK) positive tumor cells. ( A ) Reference Cell (HCT116) from a control slide. ( B ) Patient-derived circulating tumor cell (CTC) which stained positive for CK and ( C ) disseminated tumor cell (DTC) from the same patient that stainend positive against CK. CK-positive cells are visualized in pink, while nuclei are counterstained in blue. Images were processed and annotated using Aperio ImageScope software Version 12.4.6 (Leica Biosystems, Nußloch, Germany) and the figure was assembled using Microsoft PowerPoint.
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Representative microscopic images of <t>Cytokeratin</t> (CK) positive tumor cells. ( A ) Reference Cell (HCT116) from a control slide. ( B ) Patient-derived circulating tumor cell (CTC) which stained positive for CK and ( C ) disseminated tumor cell (DTC) from the same patient that stainend positive against CK. CK-positive cells are visualized in pink, while nuclei are counterstained in blue. Images were processed and annotated using Aperio ImageScope software Version 12.4.6 (Leica Biosystems, Nußloch, Germany) and the figure was assembled using Microsoft PowerPoint.
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Representative microscopic images of Cytokeratin (CK) positive tumor cells. ( A ) Reference Cell (HCT116) from a control slide. ( B ) Patient-derived circulating tumor cell (CTC) which stained positive for CK and ( C ) disseminated tumor cell (DTC) from the same patient that stainend positive against CK. CK-positive cells are visualized in pink, while nuclei are counterstained in blue. Images were processed and annotated using Aperio ImageScope software Version 12.4.6 (Leica Biosystems, Nußloch, Germany) and the figure was assembled using Microsoft PowerPoint.

Journal: International Journal of Molecular Sciences

Article Title: Disseminated Tumor Cells (DTCs) in Patients with Cervical Cancer Reveal Mesenchymal Properties and Potential Therapeutic Targets—A New Perspective?

doi: 10.3390/ijms27114875

Figure Lengend Snippet: Representative microscopic images of Cytokeratin (CK) positive tumor cells. ( A ) Reference Cell (HCT116) from a control slide. ( B ) Patient-derived circulating tumor cell (CTC) which stained positive for CK and ( C ) disseminated tumor cell (DTC) from the same patient that stainend positive against CK. CK-positive cells are visualized in pink, while nuclei are counterstained in blue. Images were processed and annotated using Aperio ImageScope software Version 12.4.6 (Leica Biosystems, Nußloch, Germany) and the figure was assembled using Microsoft PowerPoint.

Article Snippet: In the first round of staining, anti-human Pan-Cytokeratin APC-conjugated REAdye_lease antibody (Cat. No. 130-123-091, Miltenyi Biotech, Bergisch Gladbach, Germany), anti-human vimentin FITC-conjugated REAdye_lease antibody (Cat. No. 130-127-022, Miltenyi Biotech, Bergisch Gladbach, Germany) and anti-human VEGF PE-conjugated REAdye_lease antibody (Cat. No. 130-118-061, Miltenyi Biotech, Bergisch Gladbach, Germany) were applied.

Techniques: Control, Derivative Assay, Staining, Software

Positive and negative control cells for both marker panels. ( A ) HeLa (white arrows): in the first panel positive for cytokeratin (CK), Vimentin (Vim), VEGF and in the second panel positive for p16INK4A, negative for PD-L1 and CD45. ( B ) T98G (red arrows): positive for Vim. ( C ) CaSki (yellow arrows): positive for CK, Vim, VEGF, PD-L1 and p16INK4A. ( D ) MCF-7 (green arrows): positive for CK. All cell lines were negative for CD45. Only hematopoietic cells showed CD45 positive staining. Scale bar = 10 μm. Images were processed using Zeiss ZEN 3.7 software and the figure was assembled using Microsoft PowerPoint.

Journal: International Journal of Molecular Sciences

Article Title: Disseminated Tumor Cells (DTCs) in Patients with Cervical Cancer Reveal Mesenchymal Properties and Potential Therapeutic Targets—A New Perspective?

doi: 10.3390/ijms27114875

Figure Lengend Snippet: Positive and negative control cells for both marker panels. ( A ) HeLa (white arrows): in the first panel positive for cytokeratin (CK), Vimentin (Vim), VEGF and in the second panel positive for p16INK4A, negative for PD-L1 and CD45. ( B ) T98G (red arrows): positive for Vim. ( C ) CaSki (yellow arrows): positive for CK, Vim, VEGF, PD-L1 and p16INK4A. ( D ) MCF-7 (green arrows): positive for CK. All cell lines were negative for CD45. Only hematopoietic cells showed CD45 positive staining. Scale bar = 10 μm. Images were processed using Zeiss ZEN 3.7 software and the figure was assembled using Microsoft PowerPoint.

Article Snippet: In the first round of staining, anti-human Pan-Cytokeratin APC-conjugated REAdye_lease antibody (Cat. No. 130-123-091, Miltenyi Biotech, Bergisch Gladbach, Germany), anti-human vimentin FITC-conjugated REAdye_lease antibody (Cat. No. 130-127-022, Miltenyi Biotech, Bergisch Gladbach, Germany) and anti-human VEGF PE-conjugated REAdye_lease antibody (Cat. No. 130-118-061, Miltenyi Biotech, Bergisch Gladbach, Germany) were applied.

Techniques: Negative Control, Marker, Staining, Software